Vertebrate Embryogenesis

Embryological, Cellular, and Genetic Methods
Verkaufsrang528inBiologie
HardcoverGebunden
434 Seiten
Englisch
Springer, Berlinerschienen am09.02.2019
Verfügbare Formate
HardcoverKartoniert, Paperback
EUR165,84
HardcoverGebunden
EUR165,84
HardcoverGebunden
EUR181,89
This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle....mehr
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Produkt

KlappentextThis detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development.
Chapter 10, "Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos," is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
HinweisIncludes cutting-edge, fully updated techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Details
ISBN/EAN/Artikel978-1-4939-9008-5
ProduktartHardcover
EinbandartGebunden
Jahr2019
Erschienen am09.02.2019
Seiten434 Seiten
SpracheEnglisch
Illustrationen12 SW-Abb., 70 Farbabb.
Artikel-Nr.17291079

Inhalt/Kritik

Inhaltsverzeichnis
1. Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments
            Douglas W. Houston
 
2. Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos
            Thao Nguyen, Timothy J. Mitchison, and Martin Wühr
 
3. Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos
            Michael D. Sheets
 
4.
...mehr
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