Verkaufsrang544inBiologie
HardcoverGebunden
216 Seiten
Englisch
Springer, Berlinerschienen am15.03.2019
Verfügbare Formate
HardcoverKartoniert, Paperback
EUR128,39
HardcoverGebunden
EUR160,49
HardcoverGebunden
EUR106,99
This fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. The volume also examines the analysis of high-throughput sequencing data recovered from ancient specimens, which,...mehr
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Produkt

KlappentextThis fully updated second edition explores protocols that address the most challenging aspects of experimental work in ancient DNA, such as preparing ancient samples for DNA extraction, the DNA extraction itself, and transforming extracted ancient DNA molecules for sequencing library preparation. The volume also examines the analysis of high-throughput sequencing data recovered from ancient specimens, which, because of the degraded nature of ancient DNA and common co-extraction of contaminant DNA, has challenges that are unique compared to data recovered from modern specimens.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Ancient DNA: Methods and Protocols, Second Edition aims to serve both experts and beginners by presenting protocols in a manner that makes them easily accessible for everyday use in the lab.
HinweisIncludes cutting-edge, fully updated techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Details
ISBN/EAN/Artikel978-1-4939-9175-4
ProduktartHardcover
EinbandartGebunden
Jahr2019
Erschienen am15.03.2019
Seiten216 Seiten
SpracheEnglisch
Illustrationen10 SW-Abb., 5 Farbabb., 6 Farbtabellen
Artikel-Nr.17472272

Inhalt/Kritik

Inhaltsverzeichnis1. Setting Up an Ancient DNA Laboratory
Tara L. Fulton and Beth Shapiro
2. Pretreatment: Removing DNA Contamination from Ancient Bones and Teeth Using Sodium Hypochlorite and Phosphate
Petra Korlevic and Matthias Meyer
3. Pretreatment: Improving Endogenous Ancient DNA Yields Using a Simple Enzymatic Pre-Digestion Step
Hannes Schroeder, Peter de Barros Damgaard, and Morten E. Allentoft
4.
...mehr

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